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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
doi: 10.3791/63118
Figure Lengend Snippet: Confocal microscopy images showing the immobilization of cell membrane fragments with functional TrkB receptors on IAM particles. (A) Cell membrane fragments from SH-SY5Y TrkB-NULL cell line immobilized on IAM particles after incubation with BDNF, primary antibody, and fluorophore-labeled secondary antibody. (B) Cell membrane fragments from SH-SY5Y Neuroblastoma cell lines expressing TrkB immobilized on IAM particles after incubation with primary antibody and fluorophore-labeled secondary antibody without BDNF. (C) Cell membrane fragments from SH-SY5Y neuroblastoma cell lines expressing TrkB immobilized on IAM particles after incubation with BDNF, primary antibody, and fluorophore-labeled secondary antibody.
Article Snippet: Cell culture of SH-SY5Y neuroblastoma cells (TrkB and TrkB-NULL (parental) cell lines) NOTE: Cell lines (
Techniques: Confocal Microscopy, Membrane, Functional Assay, Incubation, Labeling, Expressing